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Issue 9, 2016
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A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins

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Abstract

A semi-quantitative and quantitative multi-immunochromatographic (ICA) strip detection assay was developed for the simultaneous detection of twenty types of mycotoxins from five classes, including zearalenones (ZEAs), deoxynivalenols (DONs), T-2 toxins (T-2s), aflatoxins (AFs), and fumonisins (FBs), in cereal food samples. Sensitive and specific monoclonal antibodies were selected for this assay. The semi-quantitative results were obtained within 20 min by the naked eye, with visual limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.1–0.5, 2.5–250, 0.5–1, 0.25–1 and 2.5–10 μg kg−1, and cut-off values of 0.25–1, 5–500, 1–10, 0.5–2.5 and 5–25 μg kg−1, respectively. The quantitative results were obtained using a hand-held strip scan reader, with the calculated limits of detection for ZEAs, DONs, T-2s, AFs and FBs of 0.04–0.17, 0.06–49, 0.15–0.22, 0.056–0.49 and 0.53–1.05 μg kg−1, respectively. The analytical results of spiked samples were in accordance with the accurate content in the simultaneous detection analysis. This newly developed ICA strip assay is suitable for the on-site detection and rapid initial screening of mycotoxins in cereal samples, facilitating both semi-quantitative and quantitative determination.

Graphical abstract: A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins

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Publication details

The article was received on 24 Dec 2015, accepted on 04 Feb 2016 and first published on 05 Feb 2016


Article type: Paper
DOI: 10.1039/C5NR09171C
Citation: Nanoscale, 2016,8, 5245-5253

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    A gold nanoparticle-based semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins

    D. Kong, L. Liu, S. Song, S. Suryoprabowo, A. Li, H. Kuang, L. Wang and C. Xu, Nanoscale, 2016, 8, 5245
    DOI: 10.1039/C5NR09171C

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