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Issue 15, 2016
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Genetically designed biomolecular capping system for mesoporous silica nanoparticles enables receptor-mediated cell uptake and controlled drug release

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Abstract

Effective and controlled drug delivery systems with on-demand release and targeting abilities have received enormous attention for biomedical applications. Here, we describe a novel enzyme-based cap system for mesoporous silica nanoparticles (MSNs) that is directly combined with a targeting ligand via bio-orthogonal click chemistry. The capping system is based on the pH-responsive binding of an aryl-sulfonamide-functionalized MSN and the enzyme carbonic anhydrase (CA). An unnatural amino acid (UAA) containing a norbornene moiety was genetically incorporated into CA. This UAA allowed for the site-specific bio-orthogonal attachment of even very sensitive targeting ligands such as folic acid and anandamide. This leads to specific receptor-mediated cell and stem cell uptake. We demonstrate the successful delivery and release of the chemotherapeutic agent Actinomycin D to KB cells. This novel nanocarrier concept provides a promising platform for the development of precisely controllable and highly modular theranostic systems.

Graphical abstract: Genetically designed biomolecular capping system for mesoporous silica nanoparticles enables receptor-mediated cell uptake and controlled drug release

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Supplementary files

Article information


Submitted
18 Nov 2015
Accepted
14 Feb 2016
First published
17 Feb 2016

This article is Open Access

Nanoscale, 2016,8, 8101-8110
Article type
Paper
Author version available

Genetically designed biomolecular capping system for mesoporous silica nanoparticles enables receptor-mediated cell uptake and controlled drug release

S. Datz, C. Argyo, M. Gattner, V. Weiss, K. Brunner, J. Bretzler, C. von Schirnding, A. A. Torrano, F. Spada, M. Vrabel, H. Engelke, C. Bräuchle, T. Carell and T. Bein, Nanoscale, 2016, 8, 8101
DOI: 10.1039/C5NR08163G

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    [Original citation] - Published by The Royal Society of Chemistry.

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