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Issue 25, 2016

Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

Author affiliations

Abstract

In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.

Graphical abstract: Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

Supplementary files

Article information


Submitted
10 Nov 2015
Accepted
15 Dec 2015
First published
15 Dec 2015

Nanoscale, 2016,8, 12626-12638
Article type
Paper

Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

C. Spinato, A. Perez Ruiz de Garibay, M. Kierkowicz, E. Pach, M. Martincic, R. Klippstein, M. Bourgognon, J. T. Wang, C. Ménard-Moyon, K. T. Al-Jamal, B. Ballesteros, G. Tobias and A. Bianco, Nanoscale, 2016, 8, 12626 DOI: 10.1039/C5NR07923C

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