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Issue 25, 2016
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Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

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Abstract

In the present work we have devised the synthesis of a novel promising carbon nanotube carrier for the targeted delivery of radioactivity, through a combination of endohedral and exohedral functionalization. Steam-purified single-walled carbon nanotubes (SWCNTs) have been initially filled with radioactive analogues (i.e. metal halides) and sealed by high temperature treatment, affording closed-ended CNTs with the filling material confined in the inner cavity. The external functionalization of these filled CNTs was then achieved by nitrene cycloaddition and followed by the derivatization with a monoclonal antibody (Cetuximab) targeting the epidermal growth factor receptor (EGFR), overexpressed by several cancer cells. The targeting efficiency of the so-obtained conjugate was evaluated by immunostaining with a secondary antibody and by incubation of the CNTs with EGFR positive cells (U87-EGFR+), followed by flow cytometry, confocal microscopy or elemental analyses. We demonstrated that our filled and functionalized CNTs can internalize more efficiently in EGFR positive cancer cells.

Graphical abstract: Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

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Publication details

The article was received on 10 Nov 2015, accepted on 15 Dec 2015 and first published on 15 Dec 2015


Article type: Paper
DOI: 10.1039/C5NR07923C
Citation: Nanoscale, 2016,8, 12626-12638

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    Design of antibody-functionalized carbon nanotubes filled with radioactivable metals towards a targeted anticancer therapy

    C. Spinato, A. Perez Ruiz de Garibay, M. Kierkowicz, E. Pach, M. Martincic, R. Klippstein, M. Bourgognon, J. T. Wang, C. Ménard-Moyon, K. T. Al-Jamal, B. Ballesteros, G. Tobias and A. Bianco, Nanoscale, 2016, 8, 12626
    DOI: 10.1039/C5NR07923C

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