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Issue 14, 2016
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Ultra-fast stem cell labelling using cationised magnetoferritin

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Abstract

Magnetic cell labelling with superparamagnetic iron oxide nanoparticles (SPIONs) facilitates many important biotechnological applications, such as cell imaging and remote manipulation. However, to achieve adequate cellular loading of SPIONs, long incubation times (24 hours and more) or laborious surface functionalisation are often employed, which can adversely affect cell function. Here, we demonstrate that chemical cationisation of magnetoferritin produces a highly membrane-active nanoparticle that can magnetise human mesenchymal stem cells (hMSCs) using incubation times as short as one minute. Magnetisation persisted for several weeks in culture and provided significant T2* contrast enhancement during magnetic resonance imaging. Exposure to cationised magnetoferritin did not adversely affect the membrane integrity, proliferation and multi-lineage differentiation capacity of hMSCs, which provides the first detailed evidence for the biocompatibility of magnetoferritin. The combination of synthetic ease and flexibility, the rapidity of labelling and absence of cytotoxicity make this novel nanoparticle system an easily accessible and versatile platform for a range of cell-based therapies in regenerative medicine.

Graphical abstract: Ultra-fast stem cell labelling using cationised magnetoferritin

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Supplementary files

Article information


Submitted
14 Oct 2015
Accepted
20 Jan 2016
First published
20 Jan 2016

This article is Open Access

Nanoscale, 2016,8, 7474-7483
Article type
Paper
Author version available

Ultra-fast stem cell labelling using cationised magnetoferritin

S. Correia Carreira, J. P. K. Armstrong, A. M. Seddon, A. W. Perriman, R. Hartley-Davies and W. Schwarzacher, Nanoscale, 2016, 8, 7474
DOI: 10.1039/C5NR07144E

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