Issue 7, 2016

Deciphering the CHEF-PET-ESIPT liaison mechanism in a Zn2+ chemosensor and its applications in cell imaging study

Abstract

Proper fusion of fluorescence mechanisms in a single fluorophore unit is highly desirable to obtain better sensitivity, as well as selectivity towards a particular metal ion. In this regard, a Zn2+ chemosensor exhibiting strong fluorescence through synergistic CHEF-PET-ESIPT fluorescence mechanism has not received substantial deployment. This type of chemosensor provides an ample avenue to detect metal ions with a large stokes shift and are useful in living cell imaging study. Herein, we report an unparalleled Zn2+ chemosensor (L) based on these three types of fluorescence mechanisms by condensing 2,6-diformyl-4-methylphenol and 2-hydrazinyl-4,6-dimethylpyrimidine. All the three types of mechanism were experimentally verified by UV-Vis, fluorescence and time resolved photoluminescence (TRPL) spectroscopic evaluation. The sensor L and Zn2+ formed a 1 : 1 ratio complex demonstrating a high binding constant value (Ka = 4.812 × 105 M−1) and L showed clear discrimination from the Cd2+ ion. The limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 9.727 × 10−7 (M) and 3.24 × 10−6 (M), respectively. We have also shown that cell imaging can be performed using L without many cytotoxicity concerns.

Graphical abstract: Deciphering the CHEF-PET-ESIPT liaison mechanism in a Zn2+ chemosensor and its applications in cell imaging study

Supplementary files

Article information

Article type
Paper
Submitted
07 Feb 2016
Accepted
08 Jun 2016
First published
08 Jun 2016

New J. Chem., 2016,40, 5976-5984

Deciphering the CHEF-PET-ESIPT liaison mechanism in a Zn2+ chemosensor and its applications in cell imaging study

A. Jana, B. Das, S. K. Mandal, S. Mabhai, A. R. Khuda-Bukhsh and S. Dey, New J. Chem., 2016, 40, 5976 DOI: 10.1039/C6NJ00234J

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