Regulation of collagen expression using nanoparticle mediated inhibition of TGF-β activation

Binbin Li; Tristan D. Clemons; Vipul Agarwal; Jessica Kretzmann; Michael Bradshaw; Priyanka Toshniwal; Nicole M. Smith; Shipu Li; Mark Fear; Fiona M. Wood; K. Swaminathan Iyer

doi:10.1039/C5NJ03115J

Regulation of collagen expression using nanoparticle mediated inhibition of TGF-β activation†

Binbin Li,^ab Tristan D. Clemons,^c Vipul Agarwal,^c Jessica Kretzmann,^c Michael Bradshaw,^c Priyanka Toshniwal,^c Nicole M. Smith,^c Shipu Li,^ab Mark Fear,^de Fiona M. Wood^de and K. Swaminathan Iyer*^c

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^a State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, P. R. China

^b Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan 430070, P. R. China

^c School of Chemistry and Biochemistry, The University of Western Australia, Crawley, WA 6009, Australia
E-mail: swaminatha.iyer@uwa.edu.au

^d Fiona Wood Foundation and Burn Injury Research Unit, M318, The University of Western Australia, 35 Stirling Highway, Crawley, WA, Australia

^e Burns Service of Western Australia, Royal Perth and Princess Margaret Hospitals, WA, Australia

Abstract

PXS64 is a stable mannose-6-phosphate (M6P) analogue which has been shown to be an effective anti-fibrotic agent by inhibiting the activation of latent TGF-β1. However PXS64 is insoluble in physiological conditions. Herein, we report a multifunctional poly(glycidyl methacrylate) (PGMA) polymeric nanoparticle system for intracellular delivery of PXS64 in human primary dermal scar fibroblasts. We demonstrate the efficacy of this anti-fibrotic platform by monitoring the expression of collagen production using an in vitro scar model.

New Journal of Chemistry

Regulation of collagen expression using nanoparticle mediated inhibition of TGF-β activation†

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