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Issue 1, 2017
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Surface proteomics on nanoparticles: a step to simplify the rapid prototyping of nanoparticles

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Abstract

Engineered nanoparticles for biomedical applications require increasing effectiveness in targeting specific cells while preserving non-target cells' safety. We developed a surface proteomics method for a rapid and systematic analysis of the interphase between the nanoparticle protein corona and the targeted cells that could implement the rapid prototyping of nanomedicines. Native nanoparticles entering in a protein-rich liquid medium quickly form a macromolecular structure called protein corona. This protein structure defines the physical interaction between nanoparticles and target cells. The surface proteins compose the first line of interaction between this macromolecular structure and the cell surface of a target cell. We demonstrated that SUSTU (SUrface proteomics, Safety, Targeting, Uptake) provides a qualitative and quantitative analysis from the protein corona surface. With SUSTU, the spatial dynamics of the protein corona surface can be studied. Data from SUSTU would ascertain the nanoparticle functionalized groups exposed at a destiny that could circumvent preliminary in vitro experiments. Therefore, this method could implement in the analysis of nanoparticle targeting and uptake capability and could be integrated into a rapid prototyping strategy which is a major challenge in nanomaterials science. Data are available via ProteomeXchange with the identifier PXD004636.

Graphical abstract: Surface proteomics on nanoparticles: a step to simplify the rapid prototyping of nanoparticles

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Supplementary files

Article information


Submitted
31 Aug 2016
Accepted
21 Oct 2016
First published
21 Oct 2016

Nanoscale Horiz., 2017,2, 55-64
Article type
Communication

Surface proteomics on nanoparticles: a step to simplify the rapid prototyping of nanoparticles

J. Kuruvilla, A. P. Farinha, N. Bayat and S. Cristobal, Nanoscale Horiz., 2017, 2, 55
DOI: 10.1039/C6NH00162A

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