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Issue 1, 2016
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Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced Raman spectroscopy

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Abstract

We have produced silver glyconanoparticles for the sensitive (56 ng mL−1), low volume and rapid detection of cholera toxin B-subunit (CTB) in synthetic freshwater (simulating the ion compositions of natural waters in which CTB could be found). This is achieved by monitoring the changes in surface enhanced Raman scattering (SERS) intensity of a Raman reporter bound to the glyconanoparticle surface. The particles selectively aggregate upon interaction with CTB, causing an increase in the measured SERS signal. The particles are designed to mimic the interactions involving the cell surface GM1 ganglioside and CTB. This is achieved by using a combination of polyethylene glycol linkers terminated with either galactose or sialic acid.

Graphical abstract: Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced Raman spectroscopy

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Supplementary files

Article information


Submitted
29 Jul 2015
Accepted
20 Nov 2015
First published
20 Nov 2015

This article is Open Access

Nanoscale Horiz., 2016,1, 60-63
Article type
Communication

Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced Raman spectroscopy

J. Simpson, D. Craig, K. Faulds and D. Graham, Nanoscale Horiz., 2016, 1, 60
DOI: 10.1039/C5NH00036J

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