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Issue 10, 2016
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MicroXRF tomographic visualization of zinc and iron in the zebrafish embryo at the onset of the hatching period

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Abstract

Transition metals such as zinc, copper, and iron play key roles in cellular proliferation, cell differentiation, growth, and development. Over the past decade, advances in synchrotron X-ray fluorescence instrumentation presented new opportunities for the three-dimensional mapping of trace metal distributions within intact specimens. Taking advantage of microXRF tomography, we visualized the 3D distribution of zinc and iron in a zebrafish embryo at the onset of the hatching period. The reconstructed volumetric data revealed distinct differences in the elemental distributions, with zinc predominantly localized to the yolk and yolk extension, and iron to various regions of the brain as well as the myotome extending along the dorsal side of the embryo. The data set complements an earlier tomographic study of an embryo at the pharyngula stage (24 hpf), thus offering new insights into the trace metal distribution at key stages of embryonic development.

Graphical abstract: MicroXRF tomographic visualization of zinc and iron in the zebrafish embryo at the onset of the hatching period

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Publication details

The article was received on 24 Mar 2016, accepted on 01 Aug 2016 and first published on 17 Aug 2016


Article type: Paper
DOI: 10.1039/C6MT00073H
Metallomics, 2016,8, 1122-1130

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    MicroXRF tomographic visualization of zinc and iron in the zebrafish embryo at the onset of the hatching period

    D. Bourassa, S. Gleber, S. Vogt, C. H. Shin and C. J. Fahrni, Metallomics, 2016, 8, 1122
    DOI: 10.1039/C6MT00073H

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