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Issue 3, 2016

Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

Author affiliations

Abstract

Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides.

Graphical abstract: Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

Supplementary files

Article information


Submitted
05 Aug 2016
Accepted
23 Aug 2016
First published
07 Sep 2016

Mol. Syst. Des. Eng., 2016,1, 273-277
Article type
Communication

Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

B. E. Tam, K. Sung and H. D. Sikes, Mol. Syst. Des. Eng., 2016, 1, 273 DOI: 10.1039/C6ME00073H

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