Issue 3, 2016
From the journal:

Molecular Systems Design & Engineering

Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA†

B. E. Tam,a   K. Sunga  and  H. D. Sikes*a  

* Corresponding authors

a Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA, USA
E-mail: sikes@mit.edu

Abstract

Wild-type methyl-CpG-binding domain (MBD) proteins specifically bind symmetrically methylated DNA sequences, and assays have been developed that use these proteins for profiling DNA methylation. Here, we use directed evolution in the yeast surface display format to identify a new protein variant that binds hemi-methylated CpG dinucleotides.

Graphical abstract: Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

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Article information

DOI
https://doi.org/10.1039/C6ME00073H
Article type
Communication
Submitted
05 Aug 2016
Accepted
23 Aug 2016
First published
07 Sep 2016

Mol. Syst. Des. Eng., 2016,1, 273-277

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Engineering affinity agents for the detection of hemi-methylated CpG sites in DNA

B. E. Tam, K. Sung and H. D. Sikes, Mol. Syst. Des. Eng., 2016, 1, 273 DOI: 10.1039/C6ME00073H

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