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Issue 8, 2016
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High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

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Abstract

Quantitative proteomic approaches using selected reaction monitoring (SRM) are currently limited by the difficulty in the preparation of reference standards. In this study, we demonstrat the high-throughput production of a reference peptide library using a wheat germ cell-free synthesis system to develop a large-scale SRM assay for targeted proteomics.

Graphical abstract: High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

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Supplementary files

Article information


Submitted
23 Mar 2016
Accepted
10 May 2016
First published
11 May 2016

Mol. BioSyst., 2016,12, 2389-2393
Article type
Communication

High-throughput production of a stable isotope-labeled peptide library for targeted proteomics using a wheat germ cell-free synthesis system

N. Takemori, A. Takemori, Y. Tanaka, J. Ishizaki, H. Hasegawa, A. Shiraishi and Y. Ohashi, Mol. BioSyst., 2016, 12, 2389
DOI: 10.1039/C6MB00209A

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