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Issue 24, 2016
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Enantioselective reaction monitoring utilizing two-dimensional heart-cut liquid chromatography on an integrated microfluidic chip

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Abstract

Chip-integrated, two-dimensional high performance liquid chromatography is introduced to monitor enantioselective continuous micro-flow synthesis. The herein described development of the first two-dimensional HPLC-chip was realized by the integration of two different columns packed with reversed-phase and chiral stationary phase material on a microfluidic glass chip, coupled to mass spectrometry. Directed steering of the micro-flows at the joining transfer cross enabled a heart-cut operation mode to transfer the chiral compound of interest from the first to the second chromatographic dimension. This allows for an interference-free determination of the enantiomeric excess by seamless hyphenation to electrospray mass spectrometry. The application for rapid reaction optimization at micro-flow conditions is exemplarily shown for the asymmetric organocatalytic continuous micro-flow synthesis of warfarin.

Graphical abstract: Enantioselective reaction monitoring utilizing two-dimensional heart-cut liquid chromatography on an integrated microfluidic chip

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Supplementary files

Article information


Submitted
12 Sep 2016
Accepted
03 Nov 2016
First published
08 Nov 2016

This article is Open Access

Lab Chip, 2016,16, 4648-4652
Article type
Communication

Enantioselective reaction monitoring utilizing two-dimensional heart-cut liquid chromatography on an integrated microfluidic chip

C. Lotter, E. Poehler, J. J. Heiland, L. Mauritz and D. Belder, Lab Chip, 2016, 16, 4648
DOI: 10.1039/C6LC01138A

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