Jump to main content
Jump to site search

Issue 16, 2016
Previous Article Next Article

Delivery of minimally dispersed liquid interfaces for sequential surface chemistry

Author affiliations

Abstract

We present a method for sequential delivery of reagents to a reaction site with minimal dispersion of their interfaces. Using segmented flow to encapsulate the reagents as droplets, the dispersion between reagent plugs remains confined in a limited volume, while being transmitted to the reaction surface. In close proximity to the target surface, we use a passive array of microstructures for removal of the oil phase such that the original reagent sequence is reconstructed, and only the aqueous phase reaches the reaction surface. We provide a detailed analysis of the conditions under which the method can be applied and demonstrate maintaining a transition time of 560 ms between reagents transported to a reaction site over a distance of 60 cm. We implemented the method using a vertical microfluidic probe on an open surface, allowing contact-free interaction with biological samples, and demonstrated two examples of assays implemented using the method: measurements of receptor–ligand reaction kinetics and of the fluorescence response of immobilized GFP to local variations in pH. We believe that the method can be useful for studying the dynamic response of cells and proteins to various stimuli, as well as for highly automated multi-step assays.

Graphical abstract: Delivery of minimally dispersed liquid interfaces for sequential surface chemistry

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Apr 2016, accepted on 02 Jun 2016 and first published on 29 Jun 2016


Article type: Paper
DOI: 10.1039/C6LC00473C
Citation: Lab Chip, 2016,16, 3015-3023
  • Open access: Creative Commons BY license
  •   Request permissions

    Delivery of minimally dispersed liquid interfaces for sequential surface chemistry

    N. Ostromohov, M. Bercovici and G. V. Kaigala, Lab Chip, 2016, 16, 3015
    DOI: 10.1039/C6LC00473C

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements