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Issue 16, 2016
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Graphene-based microfluidics for serial crystallography

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Abstract

Microfluidic strategies to enable the growth and subsequent serial crystallographic analysis of micro-crystals have the potential to facilitate both structural characterization and dynamic structural studies of protein targets that have been resistant to single-crystal strategies. However, adapting microfluidic crystallization platforms for micro-crystallography requires a dramatic decrease in the overall device thickness. We report a robust strategy for the straightforward incorporation of single-layer graphene into ultra-thin microfluidic devices. This architecture allows for a total material thickness of only ∼1 μm, facilitating on-chip X-ray diffraction analysis while creating a sample environment that is stable against significant water loss over several weeks. We demonstrate excellent signal-to-noise in our X-ray diffraction measurements using a 1.5 μs polychromatic X-ray exposure, and validate our approach via on-chip structure determination using hen egg white lysozyme (HEWL) as a model system. Although this work is focused on the use of graphene for protein crystallography, we anticipate that this technology should find utility in a wide range of both X-ray and other lab on a chip applications.

Graphical abstract: Graphene-based microfluidics for serial crystallography

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Article information


Submitted
04 Apr 2016
Accepted
24 May 2016
First published
24 May 2016

This article is Open Access

Lab Chip, 2016,16, 3082-3096
Article type
Paper

Graphene-based microfluidics for serial crystallography

S. Sui, Y. Wang, K. W. Kolewe, V. Srajer, R. Henning, J. D. Schiffman, C. Dimitrakopoulos and S. L. Perry, Lab Chip, 2016, 16, 3082
DOI: 10.1039/C6LC00451B

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