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Issue 9, 2016
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Elastomeric free-form blood vessels for interconnecting organs on chip systems

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Conventional blood vessel-on-a-chip models are typically based on microchannel-like structures enclosed within bulk elastomers such as polydimethylsiloxane (PDMS). However, these bulk vascular models largely function as individual platforms and exhibit limited flexibility particularly when used in conjunction with other organ modules. Oftentimes, lengthy connectors and/or tubes are still needed to interface multiple chips, resulting in a large waste volume counterintuitive to the miniaturized nature of organs-on-chips. In this work, we report the development of a novel form of a vascular module based on PDMS hollow tubes, which closely emulates the morphology and properties of human blood vessels to integrate multiple organs-on-chips. Specifically, we present two templating strategies to fabricate hollow PDMS tubes with adjustable diameters and wall thicknesses, where metal rods or airflow were employed as the inner templates, while plastic tubes were used as the outer template. The PDMS tubes could then be functionalized by human umbilical vein endothelial cells (HUVECs) in their interior surfaces to further construct elastomeric biomimetic blood vessels. The endothelium developed biofunctionality as demonstrated by the expression of an endothelial biomarker (CD31) as well as dose-dependent responses in the secretion of von Willebrand factor and nitric oxide upon treatment with pharmaceutical compounds. We believe that with their clear advantages including high optical transparency, gas permeability, and tunable elasticity matching those of native blood vessels, these free-form PDMS vascular modules can supplement bulk vascular organoids and likely replace inert plastic tubes in integrating multiple organoids into a single microfluidic circuitry.

Graphical abstract: Elastomeric free-form blood vessels for interconnecting organs on chip systems

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Publication details

The article was received on 01 Jan 2016, accepted on 01 Mar 2016 and first published on 01 Mar 2016

Article type: Paper
DOI: 10.1039/C6LC00001K
Citation: Lab Chip, 2016,16, 1579-1586

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    Elastomeric free-form blood vessels for interconnecting organs on chip systems

    W. Zhang, Y. S. Zhang, S. M. Bakht, J. Aleman, S. R. Shin, K. Yue, M. Sica, J. Ribas, M. Duchamp, J. Ju, R. B. Sadeghian, D. Kim, M. R. Dokmeci, A. Atala and A. Khademhosseini, Lab Chip, 2016, 16, 1579
    DOI: 10.1039/C6LC00001K

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