Issue 3, 2016

Handheld real-time PCR device


Here we report one of the smallest real-time polymerase chain reaction (PCR) systems to date with an approximate size of 100 mm × 60 mm × 33 mm. The system is an autonomous unit requiring an external 12 V power supply. Four simultaneous reactions are performed in the form of virtual reaction chambers (VRCs) where a ≈200 nL sample is covered with mineral oil and placed on a glass cover slip. Fast, 40 cycle amplification of an amplicon from the H7N9 gene was used to demonstrate the PCR performance. The standard curve slope was −3.02 ± 0.16 cycles at threshold per decade (mean ± standard deviation) corresponding to an amplification efficiency of 0.91 ± 0.05 per cycle (mean ± standard deviation). The PCR device was capable of detecting a single deoxyribonucleic acid (DNA) copy. These results further suggest that our handheld PCR device may have broad, technologically-relevant applications extending to rapid detection of infectious diseases in small clinics.

Graphical abstract: Handheld real-time PCR device

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Article information

Article type
18 Nov 2015
16 Dec 2015
First published
16 Dec 2015
This article is Open Access
Creative Commons BY license

Lab Chip, 2016,16, 586-592

Handheld real-time PCR device

C. D. Ahrberg, B. R. Ilic, A. Manz and P. Neužil, Lab Chip, 2016, 16, 586 DOI: 10.1039/C5LC01415H

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