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Issue 4, 2016
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Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets

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Abstract

Position-dependent gene expression in gradients of morphogens is one of the key processes involved in cellular differentiation during development. Here, we study a simple artificial differentiation process, which is based on the diffusion of genetic inducers within one-dimensional arrangements of 50 μm large water-in-oil droplets. The droplets are filled with either bacteria or cell-free gene expression systems, both equipped with genetic constructs that produce inducers or respond to them via expression of a fluorescent protein. We quantitatively study the coupled diffusion-gene expression process and demonstrate that gene expression can be made position-dependent both within bacteria-containing and cell-free droplets. By generating diffusing quorum sensing signals in situ, we also establish communication between artificial cell-free sender cells and bacterial receivers, and vice versa.

Graphical abstract: Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets

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Supplementary files

Article information


Submitted
20 Nov 2015
Accepted
11 Jan 2016
First published
12 Jan 2016

This article is Open Access

Integr. Biol., 2016,8, 564-570
Article type
Paper

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