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Issue 2, 2016
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Fully quantified spectral imaging reveals in vivo membrane protein interactions

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Abstract

Here we introduce the fully quantified spectral imaging (FSI) method as a new tool to probe the stoichiometry and stability of protein complexes in biological membranes. The FSI method yields two dimensional membrane concentrations and FRET efficiencies in native plasma membranes. It can be used to characterize the association of membrane proteins: to differentiate between monomers, dimers, or oligomers, to produce binding (association) curves, and to measure the free energies of association in the membrane. We use the FSI method to study the lateral interactions of Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), a member of the receptor tyrosine kinase (RTK) superfamily, in plasma membranes, in vivo. The knowledge gained through the use of the new method challenges the current understanding of VEGFR2 signaling.

Graphical abstract: Fully quantified spectral imaging reveals in vivo membrane protein interactions

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Supplementary files

Article information


Submitted
12 Aug 2015
Accepted
09 Jan 2016
First published
20 Jan 2016

This article is Open Access

Integr. Biol., 2016,8, 216-229
Article type
Paper

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