Issue 10, 2016

Transketolase catalysed upgrading of l-arabinose: the one-step stereoselective synthesis of l-gluco-heptulose

Abstract

Conversion of biomass using biocatalysis is likely to become a technology that contributes significantly to the future production of chemical building blocks, materials and transport fuels. Here the synthesis of a value-added chemical from L-arabinose, a major component of the carbohydrates in sugar beet pulp (SBP), in a concise and sustainable manner has been investigated. Biocatalytic conversions using transketolase variants have been developed for the efficient, scalable synthesis of a rare naturally occurring ketoheptose, L-gluco-heptulose, from L-arabinose. New active E. coli TK mutants that readily accept L-arabinose were identified using a versatile colorimetric screening assay and the reaction was performed on a preparative scale.

Graphical abstract: Transketolase catalysed upgrading of l-arabinose: the one-step stereoselective synthesis of l-gluco-heptulose

Supplementary files

Article information

Article type
Paper
Submitted
05 Nov 2015
Accepted
16 Feb 2016
First published
16 Feb 2016
This article is Open Access
Creative Commons BY license

Green Chem., 2016,18, 3158-3165

Author version available

Transketolase catalysed upgrading of L-arabinose: the one-step stereoselective synthesis of L-gluco-heptulose

F. Subrizi, M. Cárdenas-Fernández, G. J. Lye, J. M. Ward, P. A. Dalby, T. D. Sheppard and H. C. Hailes, Green Chem., 2016, 18, 3158 DOI: 10.1039/C5GC02660A

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