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Issue 5, 2016
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A prebiotic mixture improved Lactobacillus acidophilus and Bifidobacterium animalis gastrointestinal in vitro resistance in petit-suisse

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Abstract

The survival of two probiotic strains – Lactobacillus acidophilus La-5 and Bifidobacterium animalis Bb-12 – incorporated into probiotic (PC) and into synbiotic (SC, with inulin + fructooligosaccharides, respectively, at 7.5 and at 2.5 g per 100 g) petit-suisse cheese was investigated in the beginning (day 1) and at the end (28 days) of storage at 4 °C when the food products were subjected to in vitro gastrointestinal simulated assays. Species-specific quantitative real time PCR (qPCR) combined with propidium monoazide (PMA–qPCR) was employed to quantify the strains. Initial La-5 and Bb-12 populations were always above 7 log CFU g−1. The presence of the prebiotic ingredients in SC improved the Bb-12 and La-5 resistance after the 6 h assay, with higher populations in all the in vitro stages and throughout the storage period (p < 0.05), leading to equal or superior survival rates (SR) in SC of both probiotic strains, in the beginning as well as at the end of storage. The mean La-5 SR were 58% (PC) and 67% (SC), whereas the mean Bb-12 SR were 60% (PC) and 79% (SC). Our findings suggest that the addition of a prebiotic mixture in petit-suisse cheese was advantageous, since it improved both the Bb-12 and La-5 viability and tolerance under in vitro gastrointestinal simulated conditions, both in the fresh product and in the product refrigerated for 28 days.

Graphical abstract: A prebiotic mixture improved Lactobacillus acidophilus and Bifidobacterium animalis gastrointestinal in vitro resistance in petit-suisse

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Article information


Submitted
21 Dec 2015
Accepted
08 Apr 2016
First published
12 Apr 2016

Food Funct., 2016,7, 2312-2319
Article type
Paper

A prebiotic mixture improved Lactobacillus acidophilus and Bifidobacterium animalis gastrointestinal in vitro resistance in petit-suisse

M. Padilha, M. L. Villarreal Morales, A. D. S. Vieira, M. G. M. Costa and S. M. I. Saad, Food Funct., 2016, 7, 2312 DOI: 10.1039/C5FO01592H

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