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Issue 7, 2016
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Cascade enzymatic cleavage of the β-O-4 linkage in a lignin model compound

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Abstract

The β-O-4 aryl ether linkages represent about 50% of all ethers in various lignins. At least three enzymatic steps are required to break them down: a NAD+-dependent C-α dehydrogenase (such as LigD and L), a glutathione lyase that releases guaiacol (i.e., a β-etherase such as LigE and F), and a glutathione-dependent lyase (i.e., LigG). In this work the LigD, L, E, F, and G from Sphingobium sp. SYK-6 were overexpressed in E. coli and purified with high yields. After characterizing the stability and kinetic properties of LigD and L on the lignin model compound GGE (1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol) and the thermostability of all five recombinant Lig enzymes, the experimental conditions for GGE bioconversion could be optimized (i.e., pH 9.0, 25 °C, ≈0.1 mg mL−1 of each enzyme, and 0.5 mM racemic substrate). Under optimal conditions, and by recycling NADH using the L-lactate dehydrogenase–pyruvate system, GGE was fully converted into the final products 3-hydroxy-1-(4-hydroxy-3-methoxyphenyl)propan-1-one and guaiacol in <2 hours. Differently from what was previously reported, this result and chiral HPLC analyses demonstrated that LigG catalyzes the glutathione-dependent thioether cleavage of both β(R)- and β(S)-isomer intermediates produced by LigE and LigF β-etherases: this allowed, for the first time, reaching 100% conversion of GGE. Altogether, the recombinant five-enzyme Lig system represents a component well suited for a multienzymatic process, comprising well-known ligninolytic activities (such as peroxidases and laccases), devoted to transforming selected lignins into aromatic compounds as an alternative to the oil source.

Graphical abstract: Cascade enzymatic cleavage of the β-O-4 linkage in a lignin model compound

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Supplementary files

Article information


Submitted
21 Sep 2015
Accepted
02 Nov 2015
First published
23 Nov 2015

Catal. Sci. Technol., 2016,6, 2195-2205
Article type
Paper

Cascade enzymatic cleavage of the β-O-4 linkage in a lignin model compound

E. Rosini, C. Allegretti, R. Melis, L. Cerioli, G. Conti, L. Pollegioni and P. D'Arrigo, Catal. Sci. Technol., 2016, 6, 2195
DOI: 10.1039/C5CY01591J

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