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Issue 97, 2016
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Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe

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Abstract

Herein, we developed a ratiometric two-photon probe (BHS3–EGF), derived from a pH sensitive dye and epidermal growth factor (EGF), for real-time monitoring and quantitative analysis of acidic luminal pH values during endocytic pathway activity. Two-photon microscopy imaging with BHS3–EGF allows the quantitative analysis of pH distributions of single vesicles and their dynamics in receptor-mediated endocytosis in real-time.

Graphical abstract: Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe

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Supplementary files

Article information


Submitted
05 Oct 2016
Accepted
10 Nov 2016
First published
10 Nov 2016

Chem. Commun., 2016,52, 14007-14010
Article type
Communication

Real-time monitoring of vesicle pH in an endocytic pathway using an EGF-conjugated two-photon probe

C. H. Heo, M. K. Cho, S. Shin, T. H. Yoo and H. M. Kim, Chem. Commun., 2016, 52, 14007
DOI: 10.1039/C6CC08036G

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