Using a MALDI-MS based assay, the kinetic parameters for peptide glucosylation using the C. difficile toxin B glycosyltransferase domain were determined. The minimum consensus sequence for glucosylation was YXXTXFXXY and the optimal peptide found was YAPTVFDAY. Using this sequence, homogenous glucosylated proteins could be readily produced.
Y. Sun, L. M. Willis, H. R. Batchelder and M. Nitz, Chem. Commun., 2016, 52, 13024 DOI: 10.1039/C6CC06223G
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