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Issue 75, 2016
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A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

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Abstract

Directed evolution was used to improve the activity of JamB, a membrane-bound bifunctional desaturase/acetylenase. To quickly assess the protein engineering outcomes, we developed a new platform for quantifying extracellular alkyne-tagged metabolites through a fluorogenic click reaction. Random mutagenesis yielded the best JamB variant with ∼20-fold increased activity in E. coli.

Graphical abstract: A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

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Supplementary files

Article information


Submitted
21 Jul 2016
Accepted
19 Aug 2016
First published
19 Aug 2016

Chem. Commun., 2016,52, 11239-11242
Article type
Communication

A fluorogenic screening platform enables directed evolution of an alkyne biosynthetic tool

X. Zhu, P. Shieh, M. Su, C. R. Bertozzi and W. Zhang, Chem. Commun., 2016, 52, 11239
DOI: 10.1039/C6CC05990B

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