Issue 53, 2016
From the journal:

Chemical Communications

A click chemistry-based microRNA maturation assay optimized for high-throughput screening†

Daniel A. Lorenza  and  Amanda L. Garner*ab  

* Corresponding authors

a Program in Chemical Biology, University of Michigan, 210 Washtenaw Avenue, Ann Arbor, Michigan 48109, USA

b Department of Medicinal Chemistry, College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, Michigan 48109, USA
E-mail: algarner@umich.edu

Abstract

Catalytic enzyme-linked click-chemistry assays (cat-ELCCA) are an emerging class of biochemical assay. Herein we report on expanding the toolkit of cat-ELCCA to include the kinetically superior inverse-electron demand Diels–Alder (IEDDA) reaction. The result is a technology with improved sensitivity and reproducibility, enabling automated high-throughput screening.

Graphical abstract: A click chemistry-based microRNA maturation assay optimized for high-throughput screening

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Article information

DOI
https://doi.org/10.1039/C6CC02894B
Article type
Communication
Submitted
07 Apr 2016
Accepted
26 May 2016
First published
02 Jun 2016

Chem. Commun., 2016,52, 8267-8270

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A click chemistry-based microRNA maturation assay optimized for high-throughput screening

D. A. Lorenz and A. L. Garner, Chem. Commun., 2016, 52, 8267 DOI: 10.1039/C6CC02894B

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