In vivo protein allylation to capture protein methylation candidates

Yixin Zhang; Yanbo Pan; Wujun Liu; Yongjin J. Zhou; Keyun Wang; Lei Wang; Muhammad Sohail; Mingliang Ye; Hanfa Zou; Zongbao K. Zhao

doi:10.1039/C6CC02386J

In vivo protein allylation to capture protein methylation candidates†

Yixin Zhang,‡^a Yanbo Pan,‡^b Wujun Liu,^a Yongjin J. Zhou,^a Keyun Wang,^b Lei Wang,^a Muhammad Sohail,^a Mingliang Ye,^b Hanfa Zou*^b and Zongbao K. Zhao*^ac

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* Corresponding authors

^a Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China
E-mail: zhaozb@dicp.ac.cn
Fax: +86-411-884379211
Tel: +86-411-84379211

^b Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China
E-mail: hanfazou@dicp.ac.cn

^c State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, CAS, 116023 Dalian, China

Abstract

An approach combining in vivo protein allylation, chemical tagging and affinity enrichment was devised to capture protein methylation candidates in yeast S. cerevisiae. The study identified 167 hits, covering many proteins with known methylation events on different types of amino acid residues.

Chemical Communications

In vivo protein allylation to capture protein methylation candidates†

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In vivo protein allylation to capture protein methylation candidates

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