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Issue 58, 2016
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Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

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Abstract

Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

Graphical abstract: Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

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Supplementary files

Article information


Submitted
18 Jan 2016
Accepted
31 Mar 2016
First published
31 Mar 2016

This article is Open Access

Chem. Commun., 2016,52, 9133-9136
Article type
Communication
Author version available

Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

L. A. Flanagan, J. J. Wright, M. M. Roessler, J. W. Moir and A. Parkin, Chem. Commun., 2016, 52, 9133
DOI: 10.1039/C6CC00515B

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