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Issue 58, 2016
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Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

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Abstract

Naturally occurring oxygen tolerant NiFe membrane bound hydrogenases have a conserved catalytic bias towards hydrogen oxidation which limits their technological value. We present an Escherichia coli Hyd-1 amino acid exchange that apparently causes the catalytic rate of H2 production to double but does not impact the O2 tolerance.

Graphical abstract: Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

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Publication details

The article was received on 18 Jan 2016, accepted on 31 Mar 2016 and first published on 31 Mar 2016


Article type: Communication
DOI: 10.1039/C6CC00515B
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Chem. Commun., 2016,52, 9133-9136
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    Re-engineering a NiFe hydrogenase to increase the H2 production bias while maintaining native levels of O2 tolerance

    L. A. Flanagan, J. J. Wright, M. M. Roessler, J. W. Moir and A. Parkin, Chem. Commun., 2016, 52, 9133
    DOI: 10.1039/C6CC00515B

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