Issue 13, 2016
From the journal:

Chemical Communications

Hydrophobicity-induced prestaining for protein detection in polyacrylamide gel electrophoresis†

Zhe Li,a   Weijiang Guan,a   Chao Lu,*a   Xi-Rui Zhou,b   Shi-Zhong Luo,b   Ying Youc  and  Jin Ouyangc  

* Corresponding authors

a State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
E-mail: luchao@mail.buct.edu.cn
Fax: +86 010 64411957
Tel: +86 010 64411957

b College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

c College of Chemistry, Beijing Normal University, Beijing 100875, China

Abstract

An AIE fluorescent surfactant has been first used to prestain protein by ultrastrong hydrophobic interaction between fluorescent surfactants and proteins, distinguishing from the most widely used poststaining strategies by employing AIE molecules with weak hydrophobic characteristics. A mixture of proteins with variable molecular weights has been detected.

Graphical abstract: Hydrophobicity-induced prestaining for protein detection in polyacrylamide gel electrophoresis

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Article information

DOI
https://doi.org/10.1039/C5CC09377E
Article type
Communication
Submitted
12 Nov 2015
Accepted
05 Jan 2016
First published
05 Jan 2016

Chem. Commun., 2016,52, 2807-2810

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Hydrophobicity-induced prestaining for protein detection in polyacrylamide gel electrophoresis

Z. Li, W. Guan, C. Lu, X. Zhou, S. Luo, Y. You and J. Ouyang, Chem. Commun., 2016, 52, 2807 DOI: 10.1039/C5CC09377E

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