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Issue 9, 2016
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Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

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Abstract

We report the synthesis of a new far-red fluorophore, 1,3-dichloro-7-hydroxy-2H-spiro[acridine-9,1′-cyclohexane]-2′,5′-diene-2,4′-dione (DSACO), which was modified to make two esterase probes: DSACO-2-AME and DSACO-7-AME. Both probes act as “turn-on” substrates for esterases and lipases. DSACO-2-AME exhibited efficient esterase-activated fluorescence inside living cells and is a stable, far-red alternative for the widely-used fluorescein diacetate.

Graphical abstract: Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

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Supplementary files

Article information


Submitted
21 Oct 2015
Accepted
04 Dec 2015
First published
09 Dec 2015

Chem. Commun., 2016,52, 1835-1838
Article type
Communication
Author version available

Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

S. R. Levine and K. E. Beatty, Chem. Commun., 2016, 52, 1835
DOI: 10.1039/C5CC08764C

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