Jump to main content
Jump to site search

Issue 9, 2016
Previous Article Next Article

Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

Author affiliations

Abstract

We report the synthesis of a new far-red fluorophore, 1,3-dichloro-7-hydroxy-2H-spiro[acridine-9,1′-cyclohexane]-2′,5′-diene-2,4′-dione (DSACO), which was modified to make two esterase probes: DSACO-2-AME and DSACO-7-AME. Both probes act as “turn-on” substrates for esterases and lipases. DSACO-2-AME exhibited efficient esterase-activated fluorescence inside living cells and is a stable, far-red alternative for the widely-used fluorescein diacetate.

Graphical abstract: Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Oct 2015, accepted on 04 Dec 2015 and first published on 09 Dec 2015


Article type: Communication
DOI: 10.1039/C5CC08764C
Author version
available:
Download author version (PDF)
Citation: Chem. Commun., 2016,52, 1835-1838
  •   Request permissions

    Synthesis of a far-red fluorophore and its use as an esterase probe in living cells

    S. R. Levine and K. E. Beatty, Chem. Commun., 2016, 52, 1835
    DOI: 10.1039/C5CC08764C

Search articles by author

Spotlight

Advertisements