We report a facile synthetic strategy to prepare UBL–AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1–UBL–AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.
H. An and A. V. Statsyuk, Chem. Commun., 2016, 52, 2477 DOI: 10.1039/C5CC08592F
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