Issue 12, 2016
From the journal:

Chemical Communications

Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis†

Heeseon Ana  and  Alexander V. Statsyuk*a  

* Corresponding authors

a Department of Chemistry, Center for Molecular Innovation and Drug Discovery, Chemistry of Life Processes Institute, Northwestern University, Silverman Hall, 2145 Sheridan Road, Evanston, Illinois 60208, USA

Abstract

We report a facile synthetic strategy to prepare UBL–AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1–UBL–AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.

Graphical abstract: Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

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Article information

DOI
https://doi.org/10.1039/C5CC08592F
Article type
Communication
Submitted
16 Oct 2015
Accepted
06 Nov 2015
First published
06 Nov 2015

Chem. Commun., 2016,52, 2477-2480

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Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis

H. An and A. V. Statsyuk, Chem. Commun., 2016, 52, 2477 DOI: 10.1039/C5CC08592F

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