Platinum nanoparticles encapsulated metal–organic frameworks for the electrochemical detection of telomerase activity

Abstract

A simple and rapid electrochemical sensor is constructed for the detection of telomerase activity based on the electrocatalysis of platinum nanoparticle (Pt NP) encapsulated metal–organic frameworks (MOFs), which are synthesized by one-pot encapsulation of Pt NPs into prototypal MOFs, UiO-66-NH₂. Integrating with the efficient electrocatalysis of Pt@MOFs towards NaBH₄ oxidation, this biosensor shows the wide dynamic correlation of telomerase activity from 5 × 10² to 10⁷ HeLa cells mL⁻¹ and the telomerase activity in a single HeLa cell was calculated to be 2.0 × 10⁻¹¹ IU, providing a powerful platform for detecting telomerase activity.