Control of DNA origami inter-tile connection with vertical linkers

Abstract

This communication describes a new method that enables high yield assembly along both of the two-dimensional edges of DNA origami tiles by controlling the Mg²⁺ concentration; high Mg²⁺ concentrations promote linkage connections between the vertical edges of the tiles. As a demonstration, DNA origami dimers assembled from two rectangular origami along the vertical edges are used as scaffolds for the double sided assembly of gold nanoparticles with different inter-particle spacings.