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Issue 7, 2016
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A fast click–slow release strategy towards the HPLC-free synthesis of RNA

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Abstract

A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels–Alder reaction between trans-cyclooctene and tetrazine, termed ‘click-to-release’. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer.

Graphical abstract: A fast click–slow release strategy towards the HPLC-free synthesis of RNA

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Article information


Submitted
01 Jul 2015
Accepted
24 Nov 2015
First published
24 Nov 2015

Chem. Commun., 2016,52, 1405-1408
Article type
Communication
Author version available

A fast click–slow release strategy towards the HPLC-free synthesis of RNA

E. Agustin, P. N. Asare Okai, I. Khan, M. R. Miller, R. Wang, J. Sheng and M. Royzen, Chem. Commun., 2016, 52, 1405
DOI: 10.1039/C5CC05392G

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