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Issue 7, 2016
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A fast click–slow release strategy towards the HPLC-free synthesis of RNA

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Abstract

A general strategy for purification of oligonucleotides synthesized by solid phase synthesis is described. It is based on a recently developed concept involving a bio-orthogonal inverse electron demand Diels–Alder reaction between trans-cyclooctene and tetrazine, termed ‘click-to-release’. The strategy has been applied towards the synthesis and purification of a model hairpin RNA strand, as well as a 34 nt long aptamer.

Graphical abstract: A fast click–slow release strategy towards the HPLC-free synthesis of RNA

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Publication details

The article was received on 01 Jul 2015, accepted on 24 Nov 2015 and first published on 24 Nov 2015


Article type: Communication
DOI: 10.1039/C5CC05392G
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Citation: Chem. Commun., 2016,52, 1405-1408
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    A fast click–slow release strategy towards the HPLC-free synthesis of RNA

    E. Agustin, P. N. Asare Okai, I. Khan, M. R. Miller, R. Wang, J. Sheng and M. Royzen, Chem. Commun., 2016, 52, 1405
    DOI: 10.1039/C5CC05392G

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