Jump to main content
Jump to site search

Issue 48, 2016
Previous Article Next Article

Comparison of ion mobility-mass spectrometry and pulsed-field gradient nuclear magnetic resonance spectroscopy for the differentiation of chondroitin sulfate isomers

Author affiliations

Abstract

Sulfated glycosaminoglycans such as chondroitin sulfate (CS) are important, natural polysaccharides which occur in many biological tissues and possess different biotechnological and medical applications. The analysis of CS is normally based on the evaluation of the unsaturated disaccharides, which can be easily obtained by enzymatic digestion. The 4-sulfate and the 6-sulfate are the typical main products which are usually differentiated and quantified by MS/MS. Here we show that both disaccharide isomers can be readily differentiated by ion mobility-mass spectrometry (IM-MS), with the 6-sulfate isomer having a lower mobility in the gas phase. These findings are further substantiated by pulsed-field gradient (PFG) NMR, which also indicates lower mobility of the 6-sulfate isomer in solution. Based on these already well-studied isomers we provide evidence that the combination of both methods, IM-MS and (PFG) NMR, allows the differentiation of isomers in mixtures, the determination of the relative contributions of both isomers and the structural identification without the absolute need for pure, internal standards.

Graphical abstract: Comparison of ion mobility-mass spectrometry and pulsed-field gradient nuclear magnetic resonance spectroscopy for the differentiation of chondroitin sulfate isomers

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Sep 2016, accepted on 09 Nov 2016 and first published on 09 Nov 2016


Article type: Paper
DOI: 10.1039/C6AY02531E
Citation: Anal. Methods, 2016,8, 8483-8491
  • Open access: Creative Commons BY license
  •   Request permissions

    Comparison of ion mobility-mass spectrometry and pulsed-field gradient nuclear magnetic resonance spectroscopy for the differentiation of chondroitin sulfate isomers

    K. Lemmnitzer, T. Riemer, M. Groessl, R. Süβ, R. Knochenmuss and J. Schiller, Anal. Methods, 2016, 8, 8483
    DOI: 10.1039/C6AY02531E

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements