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Issue 40, 2016
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A handheld orbital mixer for processing viscous samples in low resource settings

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Abstract

Clinical diagnostics play an important role in infectious disease monitoring. Often these diseases are endemic in settings that lack laboratory resources, which limits the types of tools that are available. Mixing is a common procedure in clinical diagnostic assays and is not immune to these challenges. In this report, we characterize the efficacy of a handheld therapeutic massager that has been repurposed into a mixer. Using metal affinity functionalized magnetic beads and a histidine-tagged fluorescent label, we find that this inexpensive orbital mixer is effective over a range of clinically relevant sample volumes and viscosities. Even with larger sample volumes (>200 μL) and more viscous sample matrices (3× ηH2O), the mixer binds more than 90% of the biomarkers in solution in less than 90 seconds. In a clinical assay for the detection of Plasmodium falciparum histidine-rich protein 2, we find there is no statistical difference in performance between our inexpensive and portable orbital mixer and a standard laboratory benchtop vortexer.

Graphical abstract: A handheld orbital mixer for processing viscous samples in low resource settings

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Supplementary files

Article information


Submitted
09 Jun 2016
Accepted
26 Aug 2016
First published
01 Sep 2016

Anal. Methods, 2016,8, 7347-7353
Article type
Paper

A handheld orbital mixer for processing viscous samples in low resource settings

T. F. Scherr, H. B. Ryskoski, A. Sivakumar, K. M. Ricks, N. M. Adams, D. W. Wright and F. R. Haselton, Anal. Methods, 2016, 8, 7347
DOI: 10.1039/C6AY01636G

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