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Issue 13, 2016
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Digital droplet LAMP as a microfluidic app on standard laboratory devices

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Abstract

Digital nucleic acid amplification methods are a growing research field that allows for absolute quantification of DNA making the need of standard curves redundant. However, most of the existing digital amplification systems require specialized laboratory devices and costly investments. The required disposable cartridges are device specific and not interchangeable. Here, we present digital droplet loop-mediated isothermal amplification (ddLAMP) as a microfluidic app on standard laboratory devices. ddLAMP is implemented on a disposable polymer chip (DropChip) in the format of a standard microscope slide. After off-chip DNA denaturation, the reaction mix is emulsified in the DropChip in a mini centrifuge for 6 minutes. The DropChip is transferred to an in situ thermal cycler for 1 hour of incubation. Afterwards, a fluorescence scan in a microarray scanner is performed. The DropChip allows for absolute quantification with a dynamic range of 15–1500 DNA copies per μl. Assay conditions were optimized for ddLAMP and comparison of ddPCR and ddLAMP for genomic E. coli DNA reveals very good concordance.

Graphical abstract: Digital droplet LAMP as a microfluidic app on standard laboratory devices

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Publication details

The article was received on 29 Feb 2016, accepted on 01 Mar 2016 and first published on 03 Mar 2016


Article type: Paper
DOI: 10.1039/C6AY00600K
Anal. Methods, 2016,8, 2750-2755
  • Open access: Creative Commons BY license
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    Digital droplet LAMP as a microfluidic app on standard laboratory devices

    F. Schuler, C. Siber, S. Hin, S. Wadle, N. Paust, R. Zengerle and F. von Stetten, Anal. Methods, 2016, 8, 2750
    DOI: 10.1039/C6AY00600K

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