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Issue 12, 2016
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A BSA coated NOA81 PCR chip for gene amplification

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Abstract

Norland Optical Adhesive 81 (NOA81) is an excellent material for a microfluidic chip, but this chip has been rarely used in the field of gene amplification due to the inhibition effect on the polymerase chain reaction (PCR). In this study, a NOA81 PCR chip (NP-chip) was developed with a simple, fast fabrication method. To overcome this inhibition effect, a simple bovine serum albumin (BSA) coating method was introduced and the BSA coated pH and concentration were optimized for a better gene amplification effect. The PCR results of HLA-DRB1 indicated that the BSA coating method could greatly improve the PCR efficiency on the NP-chip. Moreover, two kinds of PCRs were performed on the NP-chip to validate the effectiveness of the BSA coating method. (i) Gene mutation of anti EB variable region gene (AEB-HC6-6) was observed under the conditions of cobalt radiation. (ii) Short Tandem Repeats (STRs) PCR was performed on the NP-chip as the multiple-primers PCR. The results showed that the NP-chip with BSA coating was able to successfully realize single and multiple primer gene amplification. With further improvement of the precise temperature control, this kind of NP-chip would be widely applied in gene amplification and promote the development of the miniature gene amplification device in point of care testing (PCOT), forensic detection, etc.

Graphical abstract: A BSA coated NOA81 PCR chip for gene amplification

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Publication details

The article was received on 11 Dec 2015, accepted on 08 Feb 2016 and first published on 22 Feb 2016


Article type: Paper
DOI: 10.1039/C5AY03233D
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Anal. Methods, 2016,8, 2584-2591

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    A BSA coated NOA81 PCR chip for gene amplification

    K. Qin, X. Lv, Q. Xing, R. Li and Y. Deng, Anal. Methods, 2016, 8, 2584
    DOI: 10.1039/C5AY03233D

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