Development of an immunochromatographic strip for the semi-quantitative and quantitative detection of biotin in milk and milk products

Abstract

An immunochromatographic strip was developed for the semi-quantitative and quantitative detection of biotin in milk and milk products. This detection system was developed based on a sensitive and specific monoclonal antibody produced in our laboratory. The semi-quantitative results, which were visually obtained in 20 min, revealed that the visual limit of detection was 2.0 μg/100 g with a cut-off value of 8.0 μg/100 g. The quantitative results, which are obtained with a strip scan reader, revealed that the calculated limit of detection was 0.32 μg/100 g with a linear range of 0.65–68 μg/100 g. Biotin contents in milk and milk products were determined by using the developed immunochromatographic strip, and the results were validated by the microbiological assay method. Our developed immunochromatographic strip system is suitable for the on-site detection and rapid screening of biotin in food samples.