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Issue 14, 2016
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The presence of a 5′-abasic lesion enhances discrimination of single nucleotide polymorphisms while inducing an isothermal ligase chain reaction

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Abstract

Lesion-induced DNA amplification (LIDA) has been employed in the detection of single nucleotide polymorphisms (SNPs). Due to the presence of the proximal abasic lesion, T4 DNA ligase exhibits greater intolerance to basepair mismatches when compared with mismatch ligation in the absence of the abasic lesion. Moreover the presence of the abasic group also results in an isothermal ligase chain reaction enabling SNP detection with great discrimination and sensitivity. Specifically, at forty minutes, the ratio of amplified product from the matched and mismatched initiated reactions are 7–12 depending on the mismatch. The ease of implementation of our method is demonstrated by real-time analysis of DNA amplification using a fluorescent plate reader.

Graphical abstract: The presence of a 5′-abasic lesion enhances discrimination of single nucleotide polymorphisms while inducing an isothermal ligase chain reaction

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Article information


Submitted
15 Mar 2016
Accepted
27 May 2016
First published
27 May 2016

Analyst, 2016,141, 4272-4277
Article type
Communication

The presence of a 5′-abasic lesion enhances discrimination of single nucleotide polymorphisms while inducing an isothermal ligase chain reaction

A. Kausar, E. A. Osman, T. Gadzikwa and J. M. Gibbs-Davis, Analyst, 2016, 141, 4272
DOI: 10.1039/C6AN00614K

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