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Issue 11, 2016
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A portable immunomagnetic cell capture system to accelerate culture diagnosis of bacterial infections

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Abstract

Bacterial infections continue to be a major cause of deaths globally, particularly in resource-poor settings. In the absence of rapid and affordable diagnostic solutions, patients are mostly administered broad spectrum antibiotics leading to antibiotics resistance and poor recovery. Culture diagnosis continues to be a gold standard for diagnosis of bacterial infection, despite its long turnaround time of 24 to 48 h. We have developed a portable immunomagnetic cell capture (iMC2) system that allows rapid culture diagnosis of bacterial pathogens. Our approach involves the culture growth of the blood samples in broth media for 6 to 8 h, followed by immunomagnetic enrichment of the target cells using the iMC2 device. The device comprises a disposable capture chip that has two chambers of 5 ml and 50 μl volume connected through a channel with a manual valve. Bacterial cells bound to antibody coated magnetic nanoparticles are swept from the 5 ml sample chamber into the 50 μl recovery chamber by moving an external magnetic field with respect to the capture chip using a linear positioner. This enables specific isolation and up to 100× enrichment of the target cells. The presence of bacteria in the recovered sample is confirmed visually using a lateral flow immunoassay. The system is demonstrated in buffer and blood samples spiked with S. typhi. The method has high sensitivity (10 CFU ml−1), specificity and a rapid turnaround time of less than 7 h, a significant improvement over conventional methods.

Graphical abstract: A portable immunomagnetic cell capture system to accelerate culture diagnosis of bacterial infections

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Supplementary files

Article information


Submitted
04 Feb 2016
Accepted
07 Apr 2016
First published
07 Apr 2016

Analyst, 2016,141, 3358-3366
Article type
Paper
Author version available

A portable immunomagnetic cell capture system to accelerate culture diagnosis of bacterial infections

S. Singh, M. Upadhyay, J. Sharma, S. Gupta, P. Vivekanandan and R. Elangovan, Analyst, 2016, 141, 3358
DOI: 10.1039/C6AN00291A

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