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Issue 18, 2016

IR and Raman imaging of murine brains from control and ApoE/LDLR−/− mice with advanced atherosclerosis

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Abstract

Confocal Raman mapping and FT-IR imaging combined with chemometric analysis was used to study the alterations in murine brain tissue induced by the development of atherosclerosis. FT-IR imaging allowed us to obtain lower spatial resolution data (∼5.5 μm) from large, representative cross-sectional brain areas, while Raman mapping provided a more detailed insight into chosen regions of interest with high spatial resolution (∼0.4 μm). A comparison of white (WM) and grey matter (GM) from control (C57BL/6J) and ApoE/LDLR−/− mice with advanced atherosclerosis revealed disease-induced changes in both: GM and WM. The alterations included an increased lipid to protein ratio and higher total content of cholesterol.

Graphical abstract: IR and Raman imaging of murine brains from control and ApoE/LDLR−/− mice with advanced atherosclerosis

Supplementary files

Article information


Submitted
15 Jan 2016
Accepted
07 Jun 2016
First published
08 Jun 2016

Analyst, 2016,141, 5329-5338
Article type
Paper

IR and Raman imaging of murine brains from control and ApoE/LDLR−/− mice with advanced atherosclerosis

K. Kochan, K. Chrabaszcz, B. Szczur, E. Maslak, J. Dybas and K. M. Marzec, Analyst, 2016, 141, 5329 DOI: 10.1039/C6AN00107F

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