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Issue 1, 2016
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A method developed to fractionate intact proteins based on capillary electrophoresis

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Abstract

Reduction in the sample complexity enables more thorough intact protein analysis using MS-based proteomics. A capillary electrophoresis method, namely the velocity gap mode of capillary electrophoresis (VGCE), is proposed to separate protein mixtures with high resolution. Although the separation mechanism of VGCE is also based on the difference of the mass-to-charge ratios of the proteins, it fractionates the sample zone into small pieces of subunits. In this way, the resolution can be dramatically improved due to less longitudinal dispersion of the sample. The effect of the new approach is evaluated by separation of three groups of reference protein mixtures, i.e. a mixture of lysozyme and BSA; a mixture of lysozyme, β-lactoglobulin, and ribonuclease A; and a mixture of cytochrome C, lysozyme, BSA, β-lactoglobulin, ribonuclease A, conalbumin, carbonic anhydrase, and hemoglobin. The results indicate that the new approach shows great potential to couple with MS for top-down analysis of complex mixtures.

Graphical abstract: A method developed to fractionate intact proteins based on capillary electrophoresis

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Article information


Submitted
18 Aug 2015
Accepted
09 Nov 2015
First published
09 Nov 2015

Analyst, 2016,141, 305-310
Article type
Paper
Author version available

A method developed to fractionate intact proteins based on capillary electrophoresis

X. Fu, H. Xiao, S. Liang, J. J. Bao, T. Li and Y. Zhang, Analyst, 2016, 141, 305
DOI: 10.1039/C5AN01680K

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