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Issue 15, 2016
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Enzyme-conjugated ZIF-8 particles as efficient and stable Pickering interfacial biocatalysts for biphasic biocatalysis

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Abstract

In this study, a proof-of-concept for constructing efficient and stable biocatalysts is reported for biphasic catalytic reactions, where enzyme-conjugated ZIF-8 particles are utilized as efficient and stable Pickering interfacial biocatalysts. During the preparation of enzyme-conjugated ZIF-8 particles, dopamine modification on the surface of ZIF-8 particles is an essential step, which cannot only manipulate the surface hydrophilicity of ZIF-8 particles but also render surface reactive groups for conjugating enzymes in a green manner. The enzyme-conjugated ZIF-8 particles can be spontaneously assembled at the water/oil interfaces through optimizing their surface hydrophilicity, which then leads to enhanced catalytic activity as a result of much lower external and internal diffusional resistances. Such Pickering interfacial catalysts can be simply recycled and positioned at the water/oil interface via relatively low-speed centrifugation (5000 rpm), thus indicating an ease of separation for the substrate (p-NPP in the oil phase), the product (p-NP in the water phase) and catalysts (as the solid phase). Moreover, as evidenced by a 13-cycle reaction and a 37 day storage test, the robust conjugation of an enzyme on the surface of the particles can effectively protect the enzyme from the organic-solvent stress, thus retaining high catalytic activity.

Graphical abstract: Enzyme-conjugated ZIF-8 particles as efficient and stable Pickering interfacial biocatalysts for biphasic biocatalysis

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Article information


Submitted
14 Jan 2016
Accepted
10 Mar 2016
First published
11 Mar 2016

J. Mater. Chem. B, 2016,4, 2654-2661
Article type
Paper

Enzyme-conjugated ZIF-8 particles as efficient and stable Pickering interfacial biocatalysts for biphasic biocatalysis

J. Shi, X. Wang, S. Zhang, L. Tang and Z. Jiang, J. Mater. Chem. B, 2016, 4, 2654
DOI: 10.1039/C6TB00104A

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