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Issue 14, 2016
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Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation

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Abstract

Tissue-specific transcription factors are key regulators of cellular differentiation. Previously, we succeeded in introducing basic helix–loop–helix tissue-specific transcription factor proteins into cells to induce cellular differentiation. Based on these results, we decided to focus on the use of tissue-specific transcription factor proteins in the construction of biomaterials. In this proof-of-concept study, we demonstrate the construction of a tissue-specific transcription factor-tethered extracellular matrix protein. Here, the tissue-specific transcription factor Olig2 was tethered to a designed artificial extracellular matrix protein via coiled-coil helix formation. Tethered Olig2 was introduced into mouse embryonic carcinoma P19 cells attached to our designed extracellular matrix protein, and was shown to exhibit the ability to induce neural differentiation.

Graphical abstract: Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation

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Publication details

The article was received on 04 Aug 2015, accepted on 10 Mar 2016 and first published on 11 Mar 2016


Article type: Paper
DOI: 10.1039/C5TB01579K
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J. Mater. Chem. B, 2016,4, 2512-2518
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    Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation

    S. Siew, M. Kaneko, M. Mie and E. Kobatake, J. Mater. Chem. B, 2016, 4, 2512
    DOI: 10.1039/C5TB01579K

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