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Issue 2, 2016
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Speeding up biomolecular interactions by molecular sledding

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Abstract

Numerous biological processes involve association of a protein with its binding partner, an event that is preceded by a diffusion-mediated search bringing the two partners together. Often hindered by crowding in biologically relevant environments, three-dimensional diffusion can be slow and result in long bimolecular association times. Similarly, the initial association step between two binding partners often represents a rate-limiting step in biotechnologically relevant reactions. We demonstrate the practical use of an 11-a.a. DNA-interacting peptide derived from adenovirus to reduce the dimensionality of diffusional search processes and speed up associations between biological macromolecules. We functionalize binding partners with the peptide and demonstrate that the ability of the peptide to one-dimensionally diffuse along DNA results in a 20-fold reduction in reaction time. We also show that modifying PCR primers with the peptide sled enables significant acceleration of standard PCR reactions.

Graphical abstract: Speeding up biomolecular interactions by molecular sledding

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Supplementary files

Article information


Submitted
18 Aug 2015
Accepted
06 Oct 2015
First published
07 Oct 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2016,7, 916-920
Article type
Edge Article
Author version available

Speeding up biomolecular interactions by molecular sledding

A. Turkin, L. Zhang, A. Marcozzi, W. F. Mangel, A. Herrmann and A. M. van Oijen, Chem. Sci., 2016, 7, 916
DOI: 10.1039/C5SC03063C

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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