Jump to main content
Jump to site search

Issue 6, 2016
Previous Article Next Article

Fluorescence resonance energy transfer-based hybridization chain reaction for in situ visualization of tumor-related mRNA

Author affiliations

Abstract

The ability to visualize tumor-related mRNA in situ in single cells would distinguish whether they are cancer cells or normal cells, which holds great promise for cancer diagnosis at an early stage. Fluorescence resonance energy transfer (FRET) and hybridization chain reactions (HCRs) were combined with amplified sense tumor-related mRNA (TK1 mRNA) in situ with high sensitivity in single cells and tissue sections. Using this strategy, each copy of the target mRNA can propagate a chain reaction of hybridization events between two alternating hairpins to form a nicked duplex that contains repeated FRET units, amplifying the fluorescent signal. The detection limit of 18 pM is about three orders of magnitude lower than that of a non-HCR method (such as the binary-probe-system). Meanwhile, due to the FRET strategy, complicated washing steps are not necessary and experimental time is sharply reduced. As far as we know, this is the first report of a fluorescence in situ hybridization (FISH) strategy that can simultaneously fulfil signal amplification and is wash-free. We believe that this FRET-based HCR strategy has great potential as a powerful tool in basic research and clinical diagnosis.

Graphical abstract: Fluorescence resonance energy transfer-based hybridization chain reaction for in situ visualization of tumor-related mRNA

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Jan 2016, accepted on 25 Feb 2016 and first published on 25 Feb 2016


Article type: Edge Article
DOI: 10.1039/C6SC00377J
Chem. Sci., 2016,7, 3829-3835
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Fluorescence resonance energy transfer-based hybridization chain reaction for in situ visualization of tumor-related mRNA

    J. Huang, H. Wang, X. Yang, K. Quan, Y. Yang, L. Ying, N. Xie, M. Ou and K. Wang, Chem. Sci., 2016, 7, 3829
    DOI: 10.1039/C6SC00377J

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements