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Issue 5, 2016
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A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

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Abstract

We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).

Graphical abstract: A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

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Supplementary files

Article information


Submitted
05 Jan 2016
Accepted
01 Feb 2016
First published
05 Feb 2016

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2016,7, 3298-3303
Article type
Edge Article
Author version available

A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

J. Rodríguez, J. Mosquera, R. García-Fandiño, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2016, 7, 3298
DOI: 10.1039/C6SC00045B

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