Issue 5, 2016
From the journal:

Chemical Science

A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves†

Jéssica Rodríguez,a   Jesús Mosquera,a   Rebeca García-Fandiño, ORCID logo a   M. Eugenio Vázquez ORCID logo *a  and  José L. Mascareñas ORCID logo *a  

* Corresponding authors

a Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
E-mail: joseluis.mascarenas@usc.es
Fax: +34 981 595 012
Tel: +34 981576541-14405

Abstract

We report the rational design of a DNA-binding peptide construct composed of the DNA-contacting regions of two transcription factors (GCN4 and GAGA) linked through an AT-hook DNA anchor. The resulting chimera, which represents a new, non-natural DNA binding motif, binds with high affinity and selectivity to a long composite sequence of 13 base pairs (TCAT-AATT-GAGAG).

Graphical abstract: A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

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Article information

DOI
https://doi.org/10.1039/C6SC00045B
Article type
Edge Article
Submitted
05 Jan 2016
Accepted
01 Feb 2016
First published
05 Feb 2016
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2016,7, 3298-3303

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A designed DNA binding motif that recognizes extended sites and spans two adjacent major grooves

J. Rodríguez, J. Mosquera, R. García-Fandiño, M. E. Vázquez and J. L. Mascareñas, Chem. Sci., 2016, 7, 3298 DOI: 10.1039/C6SC00045B

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