Jump to main content
Jump to site search

Issue 37, 2016, Issue in Progress
Previous Article Next Article

Synthesis and characterization of an enzyme-degradable zwitterionic dextran hydrogel

Author affiliations

Abstract

The interest in dextran hydrogels for soft tissue engineering has increased due to their great biocompatibility and capability to reduce nonspecific protein adsorption and cell attachment. However, these properties are not sufficient for dextran hydrogels to fulfill their role to work as a tissue engineering scaffold candidate. In addition, the scaffold should have controllable degradability and functionality to conjugate bioactive moieties to facilitate tissue growth and regeneration. Therefore, a peptide cross-linked, enzyme degradable zwitterionic carboxybetaine functionalized dextran (CB-Dex) hydrogel was synthesized and studied. After the incorporation of the carboxybetaine group into dextran, the dextran hydrogel obtained excellent antifouling properties to resist the attachment of bovine aortic endothelial cell (BAEC) adhesion with a surface density as low as 5.5 × 102 per cm2 and meanwhile CB-Dex does not compromise the low toxicity of natural dextran. In addition, the CB-Dex hydrogel was hydrolytically stable in PBS solution and can be degraded completely within 8 hours when exposed to 0.1 mg mL−1 of collagenase. Moreover, the zwitterionic hydrogel was functionalized with the cell-adhesion peptide (Arg-Gly-Asp (RGD)) and found to enhance cell adhesion with the cell coverage density increased to 1.3 × 104 per cm2. Together, these data suggest that the matrix metalloproteinase peptide cross-linked zwitterionic hydrogel with RGD would be advantageous in tissue engineering scaffold applications.

Graphical abstract: Synthesis and characterization of an enzyme-degradable zwitterionic dextran hydrogel

Back to tab navigation

Supplementary files

Article information


Submitted
07 Jan 2016
Accepted
13 Mar 2016
First published
24 Mar 2016

RSC Adv., 2016,6, 30862-30866
Article type
Communication

Synthesis and characterization of an enzyme-degradable zwitterionic dextran hydrogel

H. Wu, H. Wang, F. Cheng, F. Xu and G. Cheng, RSC Adv., 2016, 6, 30862
DOI: 10.1039/C6RA00550K

Social activity

Search articles by author

Spotlight

Advertisements