Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 22nd May 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 1, 2016
Previous Article Next Article

Selective and comprehensive characterization of the quinochalcone C-glycoside homologs in Carthamus tinctorius L. by offline comprehensive two-dimensional liquid chromatography/LTQ-Orbitrap MS coupled with versatile data mining strategies

Author affiliations

Abstract

Quinochalcone C-glycosides (QCGs) are a series of pharmacologically bioactive components chemotaxonomic for Carthamus tinctorius L. The low abundance and ubiquitous interference from flavonoid O-glycosides (FOGs) frequently hinder the systematic exposure and characterization of these QCG homologs. We herein present an offline comprehensive two-dimensional liquid chromatography/linear ion-trap quadrupole/Orbitrap mass spectrometry (2D LC/LTQ-Orbitrap MS) approach coupled with versatile data mining strategies, to systematically characterize the QCGs from C. tinctorius. Initially, an offline 2D LC system, with an orthogonality of 71% and a theoretical peak capacity of 7654, was established by combining an Acchrom XAmide column and a BEH Shield RP-18 column. Subsequently, the water extract of C. tinctorius was separated by first dimensional hydrophilic interaction liquid chromatography (HILIC) yielding twelve fractions, which were further analyzed by reversed-phase ultra-high performance liquid chromatography/LTQ-Orbitrap MS using high energy C-trap dissociation (HCD) and collision-induced dissociation (CID) in the negative ion mode. The characteristic product ion filtering of m/z 119.05 (C8H7O) in the HCD spectra, ring double bond equivalent (RDB 10–30), characteristic UV absorption around 405 nm, preferred 0,2X0 cleavage for C-glycosides, and diagnostic product ions analysis, were simultaneously employed for the structural elucidation of QCGs. Ultimately, 163 QCQ homologs were putatively characterized, and 149 are potential new ones. Particularly, nine dimers of QCG-FOG have not been previously reported. The obtained results have greatly expanded the knowledge on the structural diversity of QCGs, demonstrating the potency of the offline comprehensive 2D LC/LTQ-Orbitrap MS approach in separation and characterization of minor herbal components.

Graphical abstract: Selective and comprehensive characterization of the quinochalcone C-glycoside homologs in Carthamus tinctorius L. by offline comprehensive two-dimensional liquid chromatography/LTQ-Orbitrap MS coupled with versatile data mining strategies

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Nov 2015, accepted on 11 Dec 2015 and first published on 15 Dec 2015


Article type: Paper
DOI: 10.1039/C5RA23744K
RSC Adv., 2016,6, 495-506

  •   Request permissions

    Selective and comprehensive characterization of the quinochalcone C-glycoside homologs in Carthamus tinctorius L. by offline comprehensive two-dimensional liquid chromatography/LTQ-Orbitrap MS coupled with versatile data mining strategies

    W. Yang, W. Si, J. Zhang, M. Yang, H. Pan, J. Wu, S. Qiu, C. Yao, J. Hou, W. Wu and D. Guo, RSC Adv., 2016, 6, 495
    DOI: 10.1039/C5RA23744K

Search articles by author

Spotlight

Advertisements